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. 2002 Mar;68(3):1336–1343. doi: 10.1128/AEM.68.3.1336-1343.2002

FIG. 2.

FIG. 2.

Utilization of exogenous pyrimidines by salvage pathway mutants. Diluted suspensions of the following yeast strains were spotted onto plates with SC-HIS-URA supplemented with the indicated pyrimidines (final concentration, 20 mg/liter). Diethylstilbestrol (DES, 1 μg/liter) restores URA3 expression and de novo pyrimidine synthesis in the ura3 strain used (see text for details and Table 1). The strains and relevant genotypes are as follows: YYM8, YZGA439 (Δurk1::TRP1), YZRM12 (Δydr400w::LEU2), YZRM15 (Δurk1::TRP1 Δydr400w:: LEU2), YZRM18 (Δurk1::TRP1 Δydr400w::kanMX), and YZRM22 (p URA3). Note that urk1 ydr400 double mutants are unable to utilize uridine as the sole source of pyrimidines.