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Nucleoside hydrolase activity towards different substrates. The activity (in milliunits per milligram of protein) present in strains with a different URH1 copy number was determined (log phase; SC-LEU). The strains used were YZRM12 (Δurh1), YZRM70 (URH1), and YZRM24 (2μm PADH1-URH1).