Table 1.
Data collection andrefinement statistics for cryo-EM
| H7 HA: SA-67 | H7 HA: ING-16-36 | |
|---|---|---|
| EMD-70657 | EMD-70658 | |
| PDB-9ONZ | PDB-9OO1 | |
| Data Collection | ||
| Microscope | FEI Titan Krios | FEI Titan Krios |
| Voltage (kV) | 300 | 300 |
| Electron dose (e-/Å2) | 50 | 50 |
| Detector | Gatan K3 | Gatan K3 |
| Pixel Size (Å) | 0.899 | 0.899 |
| Defocus Range (µm) | −1.0 to −2.5 | −1.0 to −2.5 |
| Magnification | 81,000 | 81,000 |
| Reconstruction | ||
| Software | cryoSPARC | cryoSPARC |
| Particles | 155,278 | 195,885 |
| Symmetry | C3 | C3 |
| Box size (pix) | 360 | 360 |
| Resolution (Å) (FSC0.143)* | 2.77 | 2.76 |
| Refinement (Phenix) † | ||
| Protein residues | 1470 | 1470 |
| Chimera CC | 0.9 | 0.9 |
| Resolution (Å) (FSC0.5) | 3 | 2.9 |
| EMRinger Score | 3.62 | 3.94 |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.003 | 0.003 |
| Bond angles (°) | 0.554 | 0.599 |
| Validation | ||
| MolProbity score | 1.88 | 1.87 |
| Clash score | 4.52 | 4.3 |
| Rotamers outliers (%) | 0 | 0 |
| Ramachandran | ||
| Favored regions (%) | 96.3 | 97.19 |
| Disallowed regions (%) | 0 | 0 |
*Resolutions are reported according to the FSC 0.143 gold-standard criterion.
†Statistics are reported for the protein residues within the complex.