Fig. 2.

Schematic diagram of the transformation vector pPhII-YFP. The vector carries the complete phII gene including its promoter region. Exons (introns) are shown as black boxes (thin lines). Directly upstream of the TAG stop codon, a 0.7 kb fragment coding for a flexible penta-glycine spacer (cyan) and the codon-adapted coding sequence of yfp (mVenus) (yellow) were inserted in frame using an artificially introduced KpnI site. The KpnI was inserted before into the section between MluI and ClaI (asterisks). The vector backbone (dashed lines) comes from pUC18. For details, see Materials and Methods; the sequence of the vector insert is in SI Appendix, Fig. S3.