Fig. 8.

PA axis and life cycle variation in top view. (A1–G1): Computed metrics binned in $8$ equally spaced segments along the PA axis. Means are shown as dashed lines with per-bin SD reported by shaded segments. Colors correspond to developmental stages defined in Fig. 3. (A2–G2): Histograms of metrics by stage in $100$ equally spaced bins, by stage, with empirical means indicated by vertical black bars. Units are noted in parentheses, and otherwise, are dimensionless. See SI Appendix, Table S2 for numerical values of empirical means. SI Appendix, Fig. S15 and Table S5 show analogous data for distortion-corrected surface CZ3 geometry accounting for the mounting procedure (Materials and Methods). We find a maximum 5% increase in mean compartment area across stages I to IV, with minimal change in other CZ3 features.