Fig. 2.

Depletion of ITAF₄₅ restricts EMCV infection. (A) RT-qPCR quantification of EMCV RNA in control, ITAF₄₅^KO, and ITAF₄₅^KO + ITAF₄₅ infected with EMCV (Mengo strain) at a multiplicity of infection (MOI) of 10 for 7 h. (B). Plaque assay quantification of infectious EMCV produced from control, ITAF₄₅^KO, and ITAF₄₅^KO + ITAF₄₅ cells infected with EMCV (Mengo strain) at an MOI of 10 for 24 h. Datasets represent means ± s.d. (n = 3 independent biological replicates). All P values were determined by ordinary one-way ANOVA using GraphPad Prism (GraphPad Software) with Dunnett’s correction. ***P < 0.0002 and ****P < 0.0001.