Fig. 2.

Specificity of the Cys-Arg/N-degron pathway revealed through mutagenesis screening. (A) Scanning mutagenesis experiment of various N-terminal cysteine commencing peptides. For each of the indicated genes, data are presented for mutagenesis of single residues (Top) or three consecutive residues (Bottom). (B) Saturation mutagenesis of four representative substrate examples with N-terminal cysteine. For both (A) and (B), darker colors represent a greater degree of stabilization conferred by the mutation. Gene’s name is indicated at the Top of each panel and a universal scale of stabilization is provided.