Figure 2.

GPR34 silencing suppresses ATC progression. (A, B) Western blot showed siGPR34#1 and siGPR34#2 transfection efficiency in BHT101 (A) and 8305C cells (B). (C, D) The CCK8 assay represented GPR34 knockdown suppressed the proliferation of BHT101 (C) and 8305C (D) cells. (E) The colony formation assay of ATC cells with GPR34 deletion. (F) The transwell migration experiment indicated that GPR34 silencing decreased the migration capability of ATC cells. (G) BHT101 cells transfected with indicated plasmids were transplanted on nude mouse (n = 5 mouse per group). The tumors were removed and weighted after 17 days. The tumor size was assessed every 3 days. ⁣^∗p  < 0.05, ⁣^∗∗p  < 0.01, ⁣^∗∗∗p  < 0.001, ⁣^∗∗∗∗p  < 0.0001.