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. 2025 Aug 18;2025:9951847. doi: 10.1155/adpp/9951847

Table 9.

Molecular docking results of Shyobunol with selected target proteins, including protein classification, active site residues, key intermolecular interactions, and binding energies (kcal/mol).

Shyobunol complexed to 2CDU.pdb 6F86.pdb 1SMD.pdb 5NN5.pdb
Classification NAD(P)H oxidase protein (oxidoreductase) DNA gyrase-B protein (isomerase) α-Amylase protein (hydrolase) α-Glucosidase protein (hydrolase)

Active sites Ala300-
Phe245-
Lys134-
Thr9-
Ala11-
Ser115-
Glu32-
Met33-
Val81-
Asp282
Gly77-
Asp73
Unknown active sites Asp404-
Asp518-
Asp616-
Arg600-
His674

Produced intermolecular interactions Ala300-
Phe245-
Leu299-
Ile160
Gly77-
Glu50-
Pro79-
Ile78
Gln302-
Arg346-
Phe348
Val867-
Leu868-
Met363-
His717-
Leu865

Binding energies (kcal/mol) −6.48 −5.91 −6.21 −6.52

Note: Binding energies are expressed in kcal/mol. Bold values represent the most favorable (lowest) binding energies for each target, indicating stronger ligand–protein binding affinity.