Figure 7.

Bacterial species richness and venn diagrams of different taxonomic groups of the respiratory microbiota of pigs in different experimental groups: control (G1) healthy animals, infected (G2, at dpi 10, 25 and 35) with M. hyopneumoniae and treated (G3, dpi 25 and 35) with the antibiotic tylvalosin. (A) Rarefaction curves illustrating the species richness of ASVs (amplicon sequence variants) in the respiratory microbiota of pigs under different experimental conditions. The data are presented in three panels corresponding to each experimental group: control (negative control, G1, n = 4), infected (positive control, G2, n = 14) and treated (infected and treated, G3, n = 14). Each curve represents an individual animal within its respective group. The x-axis denotes the sequencing depth (number of sequences sampled), while the y-axis indicates the species richness (number of observed ASVs). Rarefaction analysis enables the evaluation of microbial diversity and sampling effort. A plateau in the curve suggests that the sequencing depth was sufficient to capture the microbial diversity present in that sample. (B) Venn diagram illustrating the shared ASVs among the experimental groups. Each circle represents a group, and the overlapping areas indicate shared ASVs. Percentages reflect the proportion of unique or shared ASVs relative to the total.