Table 1.
Mean and standard deviation values of Mycoplasma hyopneumoniae P102 gene copies (expressed as copies/µL) detected by quantitative PCR (qPCR) in laryngeal swabs, bronchoalveolar lavage fluid (BALF) and lung tissue samples collected from animals in the G2 (positive control) and G3 (infected and treated) groups.
| Group | Swab | BALF | Tissue | BALF | Tissue | BALF | Tissue | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Day 0 | 9 dpi | 16 dpi | 22 dpi | 28 dpi | 35 dpi | 10 dpi | 25 dpi | 35 dpi | |||||
| G2 | Average | 0 | 9.53E + 03 | 9.47E + 03 | 3.45E + 03 | 1.95E + 03 | 2.45E + 03 | 1.82E + 04 | 6.98E + 04 | 1.27E + 06 | 2.35E + 06 | 3.42E + 05 | 8.94E + 04 |
| DP | 0 | 1.07E + 04 | 2.45E + 04 | 1.13E + 05 | 2.76E + 04 | 1.17E + 04 | 2.12E + 02 | 4.90E + 03 | 3.83E + 05 | 2.54E + 06 | 2.15E + 06 | 1.04E + 05 | |
| Number | 0/14 | 8/14 | 12/13 | 13/13 | 11/11 | 11/11 | 1/1 | 1/1 | 2/2 | 2/2 | 11/11 | 11/11 | |
| % | 0 | 57.14 | 92.30 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | |
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| G3 | Average | 0 | 1.82E + 03 | 6.18E + 03 | 1.18E + 03 | 5.00E + 02 | 1.73E + 03 | 3.33E + 05 | 3.55E + 02 | 5.43E + 06 | 8.60E + 03 | 2.23E + 05 | 3.00E + 04 |
| DP | 0 | 9.86E + 03 | 1.26E + 05 | 8.72E + 03 | 1.15E + 04 | 3.42E + 03 | 7.07E + 03 | 9.48E + 00 | 5.68E + 06 | 1.07E + 04 | 9.80E + 05 | 4.43E + 05 | |
| Number | 0/14 | 8/14 | 12/13 | 12/13 | 11/11 | 11/11 | 1/1 | 1/1 | 2/2 | 2/2 | 11/11 | 11/11 | |
| % | 0 | 57.14 | 92.30 | 92.30 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | |
Note: Samples were collected at various time points post-infection (dpi). For each matrix and time point, the table shows: (i) mean gene copy number; (ii) standard deviation (SD); (iii) number of positive animals versus total sampled (number) and (iv) percentage of qPCR-positive animals (%). Swab samples were collected on days 0, 9, 16, 22, 28 and 35 dpi. BALF and lung tissue samples were obtained at 10, 25 and 35 dpi from subsets of euthanised animals.