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. 2025 Aug 12;16:1625944. doi: 10.3389/fendo.2025.1625944

Figure 5.

A series of scientific charts and images depicting a study on the effects of different treatments on rats. Panel A illustrates the experimental design with diagrams showing normal and high-fat diet rats, treatments, and groupings. Panels B to E display bar graphs analyzing blood glucose, serum ALP, serum OC, and gene expression levels, with significant differences indicated by asterisks. Panel F shows histological images comparing control and DOP groups. Panel G presents a line graph tracking body weight over 12 weeks. Panel H includes CT images of rat bones with accompanying bar graphs analyzing bone parameters like BMD and BV/TV.

The effect of UNC0638 and knockdown of LINC00657 on the microarchitecture of distal femur in rat DOP model. (A) Schematic flow chart of the animal assay. (B–D) Rats were fed with high glucose and high fat diet for 12 weeks, followed by STZ injection, and the blood glucose (B), serum ALP (C), and serum OCN (D) of rats in two groups were detected. (E) qPCR was used to detect expression levels of LINC00657, miR-204-5p, and IGFBP5 in two groups. (F) IHC was used for detection of G9a and H3K9me2 in DOP and control rats. Scale: 500 μm. (G,H) DOP rats were treated with UNC0638 or knockdown of LINC00657, the body weight were recorded weekly (G) and micro-CT analysis (H) was performed detect the microarchitecture of distal femur and bone indexes (BMD, BV/TV, Tb.Th, Tb.N, BS/BV, Tb.Sp and SMI). HGHF: high glucose and high fat; BMD, bone mineral density; BS/BV, bone surface/volume ratio; BV/TV, trabecular bone volume; DOP, diabetic osteoporosis; IHC, immunohistochemistry; Tb.Th, trabecular thickness; Tb.N, trabecular number; Tb.Sp, trabecular separation; SMI: structure model index. *, P<0.05.