FIG. 2.
Relative quantitation of the imazalil-resistant mutants of A. nidulans grown in the absence of imazalil compared to the wild type grown in the absence of the drug. The measured quantity of each ABC transporter gene mRNA in each of the treated samples was normalized using the Cts obtained for the tubC RNA amplifications run in the same plate. The relative amounts of ABC transporter genes and relative levels of tubulin transcripts were determined by a standard curve (i.e., Cts plotted against logarithm of the DNA copy number). The values represent the numbers of times the genes are expressed compared to that of the wild-type (wt) control grown without any drug (set at 1.00). The results are averages of three repetitions.