TABLE 1.

Primers and fluorescent probes used in this work

Primer or probe	Sequencea	Gene location (accession no.)
tubCf	5′-CGGAAACTGGCCGTCAATAT-3′	1252-1271 (M17520.1)
tubCr	5′-GGGCAAACCCGACCATAAA-3′	1297-1315 (M17520.1)
ptubC	5′-6FAM-TCCCTTCCCGCGGTTGCATTT-TAMRA-3′	1275-1295 (M17520.1)
Actinf	5′-TTCCGTGCTTGTCCCTGAAC-3′	462-481 (M22869.1)
Actinr	5′-AAGGACGGGTAGGTCGTTGC-3′	505-524 (M22869.1)
Pactin	5′-VIC-CTCGCCTGGTCTCGCCGCTGT-TAMRA-3′	483-502 (M22869.1)
AtrAf	5′-TCCCTCGCATGACAATCC-3′	1542-1560 (Z68904.1)
AtrAr	5′-GAGAGAGAATGAGGCGGAGTTG-3′	1596-1618 (Z68904.1)
pAtrA	5′-TET-ACGATGACCTAGAAGACGCAACCCTTGAC-TAMRA-3′	1565-1594 (Z68904.1)
AtrBf	5′-CGCTTTGCTATCTAACGAGTTTCAC-3′	2736-2761 (Z68905.1)
AtrBr	5′-CGGCGGGCACAAGGT-3′	2789-2804 (Z68905.1)
patrB	5′-VIC-AAGATCATTCCCTGCGTGGGCACC-3′	2764-2788 (Z68905.1)
AtrCf	5′-TGGCATCCCGCTACATGAT-3′	3954-3973 (AF082072.1)
AtrCr	5′-GGGTTGGGTTCTGGGTGAC-3′	4009-4028 (AF082072.1)
pAtrC	5′-VIC-CCGTGCCCGGATCGGCCT-TAMRA-3′	3990-4008 (AF082072.1)
AtrDf	5′-CATCCGATCTCGACGGAAAA-3′	3550-3569 (AF071411.1)
AtrDr	5′-GCGCTGGAAACAGACTTGTG-3′	3616-3597 (AF071411.1)
pabcD	5′-TET-CAACCATTGACCGCACGGGCA-TAMRA-3′	3574-3594 (AF071411.1)

^aAbbreviations: 6FAM, 6-carboxyfluorescein; TAMRA, 6-carboxy-N,N,N′,N′-tetramethylrhodamine; VIC, trademark product from Applied Biosystems; TET, 6-carboxy-4,7,2′,7′-tetrachlorofluorescein.