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. 2025 Aug 25;15:31312. doi: 10.1038/s41598-025-17044-8

Table 1.

MIC determination for selected mutants against different beta-lactams.

Mutants Ancestral allele Ancestral mutation Acquired mutations MIC (mg/L)
AMP CEP FAZ FOX AXO CTX POD FEP CAZ CZA T/C MEM IMP PTZ F/C FDC
KPC-2 > 16 > 16 > 16 16 32 16 > 32 4 16 0.5 16 4 2 > 64 2 0.25
KPC-3 H274Y > 16 > 16 > 16 16 32 16 > 32 8 64 1 32 4 2 > 64 4 0.12
KPC-33 D179Y < 8 16 < 8 8 8 4 8 < 1 128 24 2 < 1 < 0.5 < 4 < 0.12 2
KPC-2n2 KPC-2 D179Y D209V < 8 > 16 < 8 8 8 4 16 < 1 128 32 2 < 1 < 0.5 < 4 0.5 > 32
KPC-3n4 KPC-3 H274Y L169P 16 16 < 8 8 8 4 16 2 > 128 12 2 < 1 < 0.5 < 4 < 0.12 2
KPC-33n6 KPC-33 D179Y R6H F20L < 8 16 < 8 8 8 8 16 < 1 > 128 64 2 < 1 < 0.5 < 4 < 0.12 8
plasmid KPC-2n2 in naïve E. coli Top10 KPC-2 D179Y D209V < 8 > 16 < 8 8 8 4 16 < 1 128 32 2 < 1 < 0.5 < 4 0.25 2
plasmid KPC-3n4 in naïve E. coli Top10 KPC-3 H274Y L169P 16 16 < 8 8 8 4 16 2 > 128 12 2 < 1 < 0.5 < 4 < 0.12 0.5
plasmid KPC-33n6 in naïve E. coli Top10 KPC-33 D179Y R6H F20L < 8 16 < 8 8 8 8 16 < 1 > 128 64 2 < 1 < 0.5 < 4 < 0.12 2

AMP, Ampicillin ; CEP, Cephalothin, FAZ, Ceftazolin ; FOX, Cefoxitin ; AXO, Ceftriaxone ; CTX, Cefotaxime ; POD, Cefpodoxime ; FEP, Cefepime ; CAZ, Ceftazidime ; CZA; Ceftazidime-avibactam ; T/C, Ceftazdime/clavulanate ; MEM, Meropenem ; IMP, Imipenem ; PTZ, Piperacillin/tazobactam ; F/C, Cefotaxime/clavulanate; FDC, Cefiderocol.