Figure 2. Inhibition of ATPase activity by SufE.

Initial velocity plots for SufBC2D ATPase activity are shown with titrations of (A) WT SufE and (B) C51-alkylated SufE. The SufE concentrations are listed on the right side of each plot. Solid lines are from a global fit of the data to equation 1. Kinetic parameters determined from the fits are listed in Table S1. Assay conditions are described in Methods and Materials.