Figure 3. SufBCD stimulation of SufS cysteine desulfurase activity.
(A) Overall reaction scheme for persulfide transfer in the assay. The persulfide on SufS is resistant to reduction by DTT. Sulfide is shown with a blue oval to indicate the methylene blue detection assay. (B) Control reactions for SufBC2D-dependent reaction stimulation. (C) Inhibition data for Mg-ATP-containing reactions. The error bars represent the standard error for the averaged data from triplicate measurements. The solid lines are fits to the Michaelis-Menten equation. Kinetic parameters from the fits are reported in Table S2. All reaction conditions are described in Materials and Methods.