Figure 6. Genetic complementation of the ΔsufE strain with SufE point mutants.

E. coli strains carrying the pET21a empty vector or various pET21a-sufE plasmids were grown as described in the Methods section. Serial dilutions of liquid cultures were spotted on Lennox broth (LB) plates with or without 250 μM 2,2’-bipyridyl (BiPy). Growth on plates was monitored for 24 – 48 hours at 30°C. The image shown is after 40 hours growth on BiPy plates.