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Inhibition of ATPase activity by SufE. Initial velocity plots for SufBC2D ATPase activity are shown with titrations of (a) WT SufE and (b) C51-alkylated SufE. The SufE concentrations are listed on the right side of each plot. Solid lines are from a global fit of the data to equation (1). Kinetic parameters determined from the fits are listed in Table S1. Assay conditions are described in the "Methods" sections.
