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. 2002 Mar;68(3):1102–1108. doi: 10.1128/AEM.68.3.1102-1108.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 1. — Construction of the integration vector pKSWT for modulating biotin synthesis in B. subtilis. First, the B. subtilis groE promoter (P_groE) and gluconate operator (O_gnt) were inserted in pK184 containing the E. coli rrnB transcription terminator (T₁T₂) to form pKPT. Then, the downstream (bioW_D) and upstream (bioW_u) sequences of B. subtilis bioW were inserted sequentially to form pKWT. Finally, the spectinomycin resistance marker (Sp^r) was inserted as shown to generate the integration vector pKSWT.