Table 2. Example calculations for the CMVn-OC43-mRuby-Ribo-BGH-YCpBAC assembly reaction (Figure 3).

Fragment name	μg†	V (μL)	[ng/μL]	Size (bp)	pmol/μL‡	pmol	V in assembly (μL)
CMVn-OC43-WT-Ribo-BGH-YCpBAC +PmeI/I-SceI	1.64	20	82	33734	0.0037	0.02	5.41 μL (444 ng)
OC43TAR456-mRubyH2B-Ribo-BGH-YCpBAC +SalI/SgrAI	1.13	20	56	11595	0.0415	0.3	7.23 μL (405 ng)