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. 2025 Aug 20;15(16):e5422. doi: 10.21769/BioProtoc.5422
Reagent Final concentration Quantity or Volume
Yeast extract 1% w/v 5 g
Peptone 2% w/v 10 g
D-Glucose 2% w/v 10 g
Adenine hemisulfate 80 mg/L 40 mg
Type II water n/a Up to 500 mL