| Reagent | Final concentration | Quantity or Volume |
|---|---|---|
| EDTA disodium salt dihydrate | 500 mM | 18.61 g |
| Type II water | n/a | 80 mL |
| Adjust to pH 8 with 5 N NaOH to dissolve the EDTA | ||
| Type II water | n/a | Up to 100 mL |
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