Reagent	Final concentration	Quantity or Volume
D-Sorbitol	1 M	18.2 g
1 M CaCl2 solution	6.5 mM	0.6 mL
Yeast extract	0.25% w/v	0.25 g
Peptone	0.5% w/v	0.5 g
Type II water	n/a	Up to 100 mL