| Reagent | Final concentration | Quantity or Volume |
|---|---|---|
| D-Glucose | 2% w/v | 7 g |
| Yeast nitrogen base, w/o amino acids, w/ ammonium sulfate | 6.74 g/L | 2.36 g |
| Yeast synthetic drop-out medium supplement, without uracil | 1.92 g/L | 0.67 g |
| Adenine hemisulfate | 80 mg/L | 28 mg |
| Type II water | n/a | 300 mL |
| Adjust to pH 5.6 if necessary | ||
| Type II water | n/a | Up to 350 mL |
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