Reagent	Final concentration	Quantity or Volume
Zymolyase solution (Recipe 8)	0.25 mg/mL zymolyase	0.5 mL
1 M Tris-HCl (pH 7.5) solution	50 mM	0.98 mL
Type II water	n/a	13.64 mL
Glycerol	25% v/v	4.88 mL