Reagent	Final concentration	Quantity or Volume
Ficoll-400	15% w/v	3 g
500 mM EDTA solution (pH 8.0)	60 mM	2.4 mL
1 M Tris-HCl (pH 7.5) solution	20 mM	0.4 mL
2% SDS solution	0.48% v/v	4.8 mL
Bromophenol blue	0.03% w/v	6 μg
Type II water	n/a	Up to 20 mL