| Reagent | Final concentration | Quantity or Volume | ||
|---|---|---|---|---|
| 0.5 M Tris-Cl (pH 6.8) | 125 mM | 10 mL | ||
| Sodium dodecyl sulfate | 4% w/v | 1.6 g | ||
| Glycerol | 20% v/v | 8 mL | ||
| Type II water | n/a | Up to 40 mL | ||
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