| Reagent | Final concentration | Quantity or Volume |
|---|---|---|
| Tris base | 2 M | 242 g |
| Glacial acetic acid | 1 M | 57 mL |
| 0.5 M EDTA pH 8.0 | 50 mM | 100 mL |
| H2O | n/a | to 1 L |
| Total | n/a | 1 L |
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