Reagent	Final concentration	Quantity or Volume
1 M Tris-HCl pH 6.8	125 mM	1.25 mL
10% SDS	4% SDS	4 mL
Glycerol	20%	2 mL
Bromophenol blue	0.01%	0.001 g
H2O	n/a	2.75 mL
Total	n/a	10 mL