Table 8. Ligation reaction setup.
| Reagent | Volume |
|---|---|
| Ligation buffer | 2 μL |
| Linearized vector DNA (pkTol2C-EGFP) | 50 ng |
| Digested insert DNA (G.O.I) | 37.5 ng |
| T4 ligase enzyme | 1 μL |
| Nuclease-free water | Up to 20 μL |
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