Reagent	Final concentration	Quantity or Volume
1 M Tris-HCl stock solution	0.1 M	10 mL
0.5 M EDTA stock solution	0.05 M	10 mL
10% SDS stock solution	1%	10 mL
10 mg/mL Proteinase K	100 µg/mL	1 mL
Distilled water	n/a	69 mL