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HPAEC elution profiles of the reaction products of AgdB from maltose. The enzyme (2.5 μg) was incubated with 0.5% maltose in 100 μl of 40 mM acetate buffer (pH 5.5) at 45°C for 5 (B) and 60 (C) min. The reaction products were subjected to HPAEC-PAD analysis. (A) Elution profile of the standard sugars. Glucose (G), maltose (M), maltotriose (G3), maltotetraose (G4), maltopentaose (G5), isomaltose (I), panose (P), and isomaltotriose (IG3) were used as standards.
