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Purification of α-glucosidase B from A. nidulans
| Purification step | Vol (ml) | Total protein (mg) | Total activity (U) | Sp act (U/mg) | Yield (%) | Purifi- cation (fold) |
|---|---|---|---|---|---|---|
| Cell extract | 167 | 560 | 106 | 0.19 | 100 | 1 |
| DEAE-Toyopearl 650M | 35 | 68 | 62 | 0.91 | 59 | 4.8 |
| Phenyl Sepharose CL-4B | 28 | 8.2 | 30 | 3.7 | 29 | 19 |
| Resource Q | 27 | 2.4 | 23 | 9.6 | 22 | 52 |
