Fig. 2. (A) QCM-D measurement showing stepwise the formation of an SLB using DOPC-based SUVs doped with 5% of DGS-NTA lipids, and the subsequent binding of His-tagged nano (1 μM). Gray shadings indicate the addition of the indicated components, while white areas indicate the buffer addition. (B) Limiting frequency shifts obtained for the binding of nano in QCM-D measurements (in black) and the diffusion coefficient of mOrange-nano (in red) as a function of the DGS-NTA fraction in the SLBs. Black and lines are guides to the eye. (C) Fluorescence recovery after photobleaching (FRAP) images of DOPC-SLBs with 1% (left) and 10% (right) DGS-NTA functionalized with mOrange-nano before bleaching (top) and after 120 s recovery (bottom). Scale bar represents 20 μm.
