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. 2025 Aug 12;13:1620369. doi: 10.3389/fcell.2025.1620369

FIGURE 2.

Grouped image with five panels related to the expression and effects of Jpx. Panel A shows a bar graph of relative expression of Jpx, with significant increases marked by asterisks. Panel B displays a bar chart of mRNA expression for ANP, BNP, and α-SKA, highlighting significant changes with asterisks. Panel C presents a bar graph showing protein/DNA ratios. Panel D consists of fluorescent microscopic images, comparing FITC, DAPI, and merged stains across four conditions. Panel E is a bar chart depicting relative cell area, with significant differences indicated by asterisks.

Overexpression of Jpx promotes hypertrophy of H9c2 cells. (A) The transfection efficiency of Jpx was detected by qRT-PCR after H9c2 cells were starved for 4 h and then transfected with testosterone, empty pcDNA3.1, or pcDNA3.1-Jpx. (B) The relative expression of ANP, BNP, and α-SKA in H9c2 cells after transfection. (C) The protein/DNA ratio of H9c2 cells after transfection. (D,E) The area of H9c2 cells after transfection was detected by immunofluorescence staining. Scale, 200 μm *P < 0.05, **P < 0.01, ***P < 0.001. T, testosterone.