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. 2025 Aug 12;13:1620369. doi: 10.3389/fcell.2025.1620369

FIGURE 3.

Bar graphs and scatter plots depict relative expression levels of various microRNAs, including miR-145-5p, miR-30c-5p, miR-29b-3p, and miR-322-5p, with significant differences shown in comparison to controls. Correlation coefficients and p-values indicate the strength of these relationships. Two bar graphs illustrate luciferase activity changes, showing increased activity with miR-322-5p and decreased activity with miR-145-5p. A diagram depicts the luciferase reporter construct with pGK promoter and Jpx, emphasizing sequences targeted by miR-145-5p and miR-322-5p.

Jpx targets miR-145-5p. (A) qRT-PCR results of the expression of candidate miRNAs in absolute ethanol- (control) or testosterone-treated H9c2 cells. (B–E) Relative expression correlation analysis between miRNAs (including miR-145-5p, miR-30c-5p, miR-29b-3p, and miR-322-5p) and Jpx in H9c2 cells after Jpx overexpression. (F,G) Luciferase reporter assays were conducted to confirm the interaction between Jpx and miR-322-5p/miR-145-5p. (H) The structures of the Luc-Jpx-miR-145-5p and Luc-Jpx-miR-322-5p dual luciferase reporter plasmids. *P < 0.05, **P < 0.01, ***P < 0.001. T, testosterone.