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qRT- PCR primer sequences.
| Gene name | Primers for qRT-RCR (5′to 3′) |
|---|---|
| ANP | F-ATCCCGTATACAGTGCGGTG R-GCACCTCAGAGAGGGAGCTA |
| BNP | F-AGCTGCTGGAGCTGATAAGAG R-GGCGCTGTCTTGAGACCTAA |
| α-SKA | F-ATTGTGCACCGCAAATGCTT R-CCCTGCAACCATAGCACGAT |
| Nfatc3 | F-TACTGTTTCCCAAGGCCCAG R-GGAGAGAAGCAGTCAGAGCA |
| Jpx | F-GCTGGTCCTATCACAGTAGAGC R-TGGACTACCCTTGAGTCTGGA |
| GAPDH | F-AGTGCCAGCCTCGTCTCATA R-TTCTCAGCCTTGACTGTGCC |
| miR-145-5p | F-GTCCAGTTTTCCCAGGAATCCCT |
| mRQ 3′primer | included in kit |
| U6 | included in kit |
