Skip to main content
. 2025 Aug 12;16:1630311. doi: 10.3389/fimmu.2025.1630311

Figure 2.

Comparison of NC and KCTD10 effects in A549 cells and tumors. (A) Fluorescent microscopy showing increased expression of green fluorescent protein in KCTD10 group. (B) Western blot analysis indicating KCTD10 and tubulin levels. (C) Colony formation assay with a significant reduction in colonies for KCTD10. (D, E) Images and graphs showing tumors with reduced volume and weight in KCTD10. (F) Histological analysis revealing cellular changes. (G, H) Migration and invasion assays showing reduced cell numbers in KCTD10. (I, J) Lung nodules and tumor burden significantly decreased in KCTD10. (K) Western blot of adhesion and signaling proteins. (L) Immunohistochemistry displaying differences in protein expression between groups.

Overexpression of KCTD10 inhibits lung cancer growth and metastasis in vitro and in vivo. (A) Fluorescence image showing the efficiency of lentiviral infection in A549 cells. (B) Western blot analysis confirming KCTD10 overexpression in A549 cells. (C) Colony formation assays demonstrating the effect of KCTD10 overexpression on cell growth. (D, E) Effects of KCTD10 on the weight and volume of subcutaneous A549 tumors (n=4/group). (F) Effects of KCTD10 on cell morphology of A549 subcutaneous tumors. (G, H) Transwell assays evaluating the effect of KCTD10 overexpression on A549 cell migration and invasion. (I, J) Effect of KCTD10 overexpression on lung colonization of A549 cells injected via the tail vein (n=4). 5x105 A549 cells and KCTD10-overexpressing A549 cells were injected. (K, L) Western blot and IHC analysis of EMT-related gene expression. ***P< 0.001.