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. 2025 Aug 12;16:1630311. doi: 10.3389/fimmu.2025.1630311

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Copyright © 2025 Yin, Long, Zhou, Ouyang, Wang, He, Su, Li, Ding and Xiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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A scientific figure presenting multiple panels of experimental data. Panel A shows a gel electrophoresis result for A549 cells. Panel B depicts a Western blot analysis for KCTD10 and Tubulin across different siRNA treatments. Panels C, D, K, and L present Kaplan-Meier survival plots for lung cancer overall and post-progression survival. Panel E and M feature scatter plots correlating METTL14 and YTHDF2 with KCTD10 expression. Panels F and N show bar graphs of relative luciferase activity. Panel G and O demonstrate RT-PCR results. Panels H and P display line graphs of KCTD10 expression over time. Panels I, J, and Q provide Western blot analyses of various proteins. — METTL14 and YTHDF2 mediates m⁶A modification of KCTD10 and enhances its mRNA stability. (A) MeRIP detecting m⁶A modification of KCTD10 CDS. (B) KCTD10 protein levels following m⁶A-related interfering RNAs. (C, D) Correlation between METTL14 expression and overall/post-progression survival in lung cancer patients using Kaplan-Meier Plotter survival analysis. (E) Correlation between METTL14 and KCTD10 expression in lung cancer using the GEPIA database. (F) Effects of METTL14 on the luciferase reporter activity of KCTD10. (G) RIP analysis detecting METTL14 binding to the predicted modification site of KCTD10. (H) qPCR analysis of KCTD10 RNA stability following METTL14 knockdown. (I) Western blot analysis of KCTD10 and related protein expression in A549 cells following METTL14 interference. (J) KCTD10 protein expression after m⁶A-related RNA interference. (K, L) Correlation between YTHDF2 expression and patient survival. (M) The GEPIA database analyzing the correlation between YTHDF2 and KCTD10 expression. (N) Effect of YTHDF2 knockdown on luciferase reporter activity of KCTD10. (O) RIP analysis of YTHDF2 binding to predicted modification sites of KCTD10 in A549 cells. (P) qPCR analysis of KCTD10 RNA stability following YTHDF2 knockdown. (Q) Western blot analysis of KCTD10 and downstream gene expression following YTHDF2 knockdown. *P < 0.05, **P < 0.01, ***P < 0.001.