Table 3.
Results of the within laboratory validation study
| No. | Test chemicals | Lab 1 | Lab 2 | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1st qualified test | 2nd qualified test | 3rd qualified test | 1st qualified test | 2nd qualified test | 3rd qualified test | ||||||||||||||
| CDR | LDR | Result | CDR | LDR | Result | CDR | LDR | Result | CDR | LDR | Result | CDR | LDR | Result | CDR | LDR | Result | ||
| 1 | Lauryl gallate | 3.8 | 58.1 | S | 4.6 | 73.2 | S | - | - | - | 16.2 | 91.6 | S | 12.7 | 94.5 | S | 12.3 | 96.2 | S |
| 2 | Chloramine T trihydrate | 96.3 | 97.5 | S | 97.6 | 97.4 | S | 98 | 96.6 | S | 92.9 | 96.8 | S | 97.1 | 94.2 | S | 97.2 | 95.1 | S |
| 3 | 4-(Methylamino)phenol sulfate | 97.2 | 88.8 | S | 99.4 | 81.3 | S | 94.4 | 83.7 | S | 97.7 | 99.9 | S | 99.8 | 99.5 | S | 97.0 | 99.6 | S |
| 4 | 2-Mercaptobenzothiazole | 35 | 9.3 | S | 33.6 | 10.9 | S | 24.7 | 6.5 | S | 35.5 | 4.9 | S | 33.6 | 6.0 | S | 43.5 | 7.0 | S |
| 5 | Benzyl salicylate | 4.4 | 3.4 | NS | 1.9 | 4.4 | NS | 0.7 | 4.2 | NS | 4.0 | 1.1 | NS | 0.7 | 4.2 | NS | 3.2 | 4.6 | NS |
| 6 | Cinnamaldehyde | 62 | 18.9 | S | 59.3 | 13.5 | S | 60.2 | 14.6 | S | 25.2 | 1.1 | S | 51.5 | 5.5 | S | 49.6 | 6.7 | S |
| 7 | Imidazolidinyl urea | 0 | 53.6 | S | 0 | 39.5 | S | 0 | 37.8 | S | 0.0 | 42.7 | S | 2.2 | 37.2 | S | 0.0 | 41.7 | S |
| 8 | Ethyl acrylate | 99.7 | 16.2 | S | 100 | 10.9 | S | 98.6 | 6 | S | 85.5 | 91.5 | S | 24.3 | 0.8 | S | 30.9 | 3.7 | S |
| 9 | Salicylic acid | 2.4 | 7.1 | NS | 1 | 17.1 | S | 1.2 | 4.7 | NS | 3.3 | 8.9 | NS | 6.4 | 2.6 | NS | 6.8 | 2.9 | NS |
| 10 | Benzyl alcohol | 1.6 | 3.3 | NS | 4.6 | 2 | NS | 1 | 2.9 | NS | 1.9 | 4.8 | NS | 0.0 | 4.5 | NS | 3.3 | 5.5 | NS |
| 11 | Glycerol | 0.4 | 0.6 | NS | 2.7 | 8 | NS | 0.9 | 1.3 | NS | 0.1 | 4.6 | NS | 2.9 | 2.1 | NS | 1.9 | 4.4 | NS |
| 12 | Isopropanol | 3.8 | 1.1 | NS | 5.1 | 0.8 | NS | 1.2 | 2 | NS | 2.0 | 5.2 | NS | 4.2 | 3.5 | NS | 5.8 | 3.9 | NS |
| Reproducibility | 83.3% | 100% | |||||||||||||||||
| No. | Test chemicals | Lab 3 | LLNA result | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1st qualified test | 2nd qualified test | 3rd qualified test | |||||||||
| CDR | LDR | Result | CDR | LDR | Result | CDR | LDR | Result | |||
| 1 | Lauryl gallate | 51.5 | 87.2 | S | 48.7 | 88.3 | S | 56.9 | 83.8 | S | S |
| 2 | Chloramine T trihydrate | 99.2 | 93.6 | S | 99.2 | 96.8 | S | 99.3 | 95.8 | S | S |
| 3 | 4-(Methylamino)phenol sulfate | 99.4 | 99.3 | S | 96.5 | 99.8 | S | 93.5 | 99.9 | S | S |
| 4 | 2-Mercaptobenzothiazole | 43.2 | 2.5 | S | 36.9 | 0 | S | 30.4 | 0 | S | S |
| 5 | Benzyl salicylate | 2.4 | 1.3 | NS | 0.9 | 0 | NS | 1.3 | 4.2 | NS | S |
| 6 | Cinnamaldehyde | 56.1 | 0 | S | 49.9 | 0 | S | 57.3 | 6.3 | S | S |
| 7 | Imidazolidinyl urea | 0 | 31.5 | S | 0 | 39.3 | S | 0 | 37.2 | S | S |
| 8 | Ethyl acrylate | 30.9 | 0 | S | 30 | 0 | S | 22.8 | 0 | S | S |
| 9 | Salicylic acid | 3.7 | 3.7 | NS | 0.2 | 0 | NS | 2.3 | 0 | NS | NS |
| 10 | Benzyl alcohol | 0.1 | 1.4 | NS | 2 | 0 | NS | 0.5 | 0 | NS | NS |
| 11 | Glycerol | 25.1 | 0.8 | S | 26.7 | 0 | S | 0.4 | 0 | NS | NS |
| 12 | Isopropanol | 0.3 | 0.3 | NS | 17.8 | 0 | NS | 0.1 | 0 | NS | NS |
| Reproducibility | 83.3% | ||||||||||
CDR cysteine depletion rate, LDR lysine depletion rate, S sensitizer, NS non-sensitizer