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. 2025 Aug 25;16:1608. doi: 10.1007/s12672-025-03483-2

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© The Author(s) 2025

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 2 — Identification of genes related to cholesterol metabolism in THCA patients. A Volcano maps showed differential expression of 93 genes related to cholesterol metabolism in normal and THCA tumor tissues. Red dots represent genes up-regulated in tumor tissue, and blue dots represent down-regulated genes in tumor tissue. B Univariate Cox regression analysis showed that ten genes related to cholesterol metabolism were significantly correlated with THCA prognosis. C and D Multivariate Cox regression and Lasso analysis of 93 cholesterol metabolism-related genes. E Signature genes and risk coefficients of prognostic models obtained by Lasso analysis