Table 1.

PCR primers for amplification of virulence and antibiotic resistance genes from MRSA strains associated with bloodstream infections in sickle cell disease (SCD) patients, Riyadh, Saudi Arabia

Gene Target*	5′—Oligo Seq—3′	Product Size (bp)	Annealing Temp. (°C)	Reference
Toxic virulence genes
pvl-R	TCTGCCATATGGTCCCCAACCA	894	60	(Paniagua-Contreras et al. 2012)
pvl-F	TGCCAGACAATGAATTACCCCCATT
sea-R	TGGTGTACCACCCGCACATTGA	252	60
sea-F	TTGCAGGGAACAGCTTTAGGCAATC
sec-R	CGCCTGGTGCAGGCATCATATC	602	62
sec-F	CCCTACGCCAGATGAGTTGCACA
tst-R	CCAATAACCACCCGTTTTATCGCTTG	306	64
tst-F	AGCCCTGCTTTTACAAAAGGGGAAAA
hlg-R	CGCCTGCCCAGTAGAAGCCATT	306	64
hlg-F	TTGGCTGGGGAGTTGAAGCACA
hla-R	CCAATCGATTTTATATCTTTC	744	53	(Tavares et al. 2014)
hla-F	CGAAAGGTACCATTGCTGGT
Antibiotic resistance genes
mecA-R	CTAGTCCATTCGGTCCA	314	55	(Duran et al. 2012)
mecA-F	CCTAGTAAAGCTCCGGAA
tetK-R	GTAGTGACAATAAAC CTCCTA	360	54
tetK-F	GTAGCGACAATAGGTAATAGT
ermC-R	TAATCG TGGAATACGGGTTTG	299	54
ermC-F	AAT CGTCAA TTCCTG CAT GT
ermA-R	TTC GCAAATCCCTTCTCAAC	190	58
ermA-F	AAGCGGTAAACCCCTCTG A
tetM-R	CATATGTCCTGG CGTGTCTA	158	54	(Duran et al. 2012)
tetM-F	AGTGGAGCGATTACAGAA
16S rDNA gene
16S rDNA-R	AAG GAG GTG ATC CAG CCG CA	1500	52	(Lane 1991)
16S rDNA-F	AGA GTT TGA TCC TGG CTC AG

* Gene Target = This column lists the specific virulence, toxin, or antibiotic resistance gene that the primers are designed to amplify. 5′—Oligo Seq—3′ = This is the sequence of the oligonucleotide primer, shown from its 5-prime to 3-prime end. Product Size (bp) = This indicates the expected length, in base pairs, of the DNA fragment that will be generated or amplified by PCR. Annealing Temp. (°C) = This is the specific temperature at which the PCR primers bind (or"anneal") to their complementary sequences on the single-stranded DNA template. Reference = This column is for the source or publication from which the primer information was obtained