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. 2025 Aug 12;13:1498141. doi: 10.3389/fbioe.2025.1498141

FIGURE 3.

Illustration showing the process of creating organotypic tissue slices from healthy organs and tumors, both from mice and humans. The tissue slices are cultured using three methods: floating slice culture, filter-supporting slice culture, and 3D-TSC. Arrows indicate the progression from organ to culture method.

Organotypic tissue slice culture method. Normal or tumor tissue is obtained from experimental animals or humans, cut into thin slices, and placed in a dish for culture. Floating slice culture is placed directly in a culture dish. Filter-supporting slice culture is first placed on a filter and then placed in a culture dish. 3D-TSC is first wrapped slice with collagen, then placed in the culture inserts, and finally placed in a culture dish.