Table 2.
Classification table of metabolic pathways.
| Level | Super class | Pathway | Hit Metabolite Count |
Pathway analysis p-value | Enrichment analysis p-value | Impact | KEGG ID | LC/GC-MS Results |
MR Results |
|---|---|---|---|---|---|---|---|---|---|
| I | Amino Acid Metabolism | Glycine, serine and threonine metabolism | 8 | <0.01 | <0.01 | 0.29 | C00065,C00114,C02291,C01005,C00258,C00097,C03508,C00022 | ↑ | ↓ |
| I | Amino Acid Metabolism | Cysteine and methionine metabolism | 5 | <0.01 | <0.01 | 0.30 | C02291,C00065,C00097,C01005,C00022 | ↑ | ↓ |
| II | Amino Acid Metabolism | Alanine, aspartate and glutamate metabolism | 4 | <0.01 | <0.01 | 0.20 | C00041,C00025,C00022,C00042 | ↓ | ↓ |
| II | Lipid Metabolism | Glycerophospholipid metabolism | 4 | 0.01 | 0.14 | 0.22 | C00157,C04230,C00114,C00093 | ↓ | ↓ |
| II | Nucleotide Metabolism | Purine metabolism | 4 | 0.01 | <0.01 | 0.21 | C00385,C00020,C00130,C00387 | ↑ | / |
| III | Amino Acid Metabolism | Glutathione metabolism | 4 | <0.01 | <0.01 | 0.04 | C00097,C00025 C01879 C00315 | ↑ | ↑ |
| III | Vitamin and Cofactor Metabolism | One carbon pool by folate | 4 | <0.01 | 0.15 | 0.06 | C00114,C00065,C02291,C00097 | ↑ | ↓ |
| III | Carbohydrate Metabolism | Butanoate metabolism | 2 | 0.05 | 0.04 | 0 | C00025,C00042 | ↓ | ↑ |
| III | Lipid Metabolism | Sphingolipid metabolism | 4 | <0.01 | <0.01 | 0.06 | C06124,C00319,C00065,C12144 | ↓ | / |
| III | Amino Acid Metabolism | Arginine and proline metabolism | 3 | <0.01 | <0.01 | 0.07 | C00315,C00025,C00022 | / | ↑ |
| III* | Lipid Metabolism | alpha-Linolenic acid metabolism | 2 | 0.01 | 0.03 | 0.33 | C00157,C06427 | / | / |
| III* | Carbohydrate Metabolism | Glyoxylate and dicarboxylate metabolism | 4 | <0.01 | <0.01 | 0.09 | C02557,C00065,C00025,C00258,C00022 | ↑ | / |
*Pathways were Classified into I,II,III categories strictly according to the Classification criteria in the study. III* represents the paradoxical phenomenon of the expression of metabolites contained in this pathway, that is, the rise and fall simultaneously. KEGG IDs were obtained from the KEGG database.
The LC/GC-MS Results ↑ indicated that the expression of metabolites was increased ↓ and decreased expression of metabolites. In MR Results, ↑ it represents a positive correlation with metabolites, and vice versa ↓ represents a negative correlation;/represents no relevant trend was found.