Table 2.
Reagents for OCR and ECAR.
| Reagents | Stock concentration | Final concentration | Catalogue number |
|---|---|---|---|
| OCR | |||
| Calibration solution | — | — | Seahorse, 100840-000 |
| XF Assay medium | — | — | Seahorse, 102353-100 |
| Glucose | 2.5M | 2.5 mM | Sigma, G7021 |
| L-Glutamine | x100 | 2 mM | Sigma, G8540 |
| Oligomycin | 10 mM/DMSO | 1 μM | Sigma, 75351 |
| FCCP | 10 mM/DMSO | 1 μM | Sigma, C2920 |
| Antimycin | 10 mM/DMSO | 1 μM | Sigma, A8674 |
| Rotenone | 10 mM/DMSO | 1 μM | Sigma, R8875 |
| ECAR | |||
| Calibration solution | — | — | Seahorse, 100840-000 |
| XF Assay medium | — | — | Seahorse, 102353-100 |
| Glucose | 2.5M | 2.5 mM | Sigma, G7021 |
| L-Glutamine | 200 mM/PBS | 2 mM | Sigma, G8540 |
| Oligomycin | 10 mM/DMSO | 1 μM | Sigma, 75351 |
| 2-Deoxy-Glucose | 10 mM/DMSO | 1 μM | Sigma, D8375 |