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. 2025 Aug 12;12:1562641. doi: 10.3389/fvets.2025.1562641

Table 13.

Effects of THY, THEO, and other THY-containing EOs on hemato-biochemical indices in animal models.

Species THY/Thyme formulation Dose Observed effects References Year
Rats THEO Not specified Decrease in RBC, Hb, lymphocyte%, and MCHC; increase in WBC, monocytes, granulocytes, AST, ALT, urea, creatinine, albumin, and globulin; amelioration of Toxocara-induced changes (118) 2013
THY 30 mg/100 g bw Reduces ALT and AST levels in hydrocortisone-induced damage animals (106) 2015
Rabbits THY 3–6 mg/kg Reduces cholesterol, triacylglycerol, LDL; increases HDL with high-dose THY (112) 2016
THY 250 mg/kg feed, 21 days Decreases ALP (p = 0.0183) and cholesterol (p = 0.0228); increases urea (p = 0.0079) upon withdrawal; decreases triglycerides in THY withdrawal group (120) 2020
Weaned pigs Lippia origanoides-E. caryophyllata EOs (47.5% THY) 67 mg/kg and 75 mg/kg blends No changes in cortisol levels; increases plasma citrulline and disaccharidase activities in post-weaning stressed pigs (20) 2021
Goats THEO 0.5–2 ml/kg feed No significant effect on ALT; reduces AST at 0.5 ml/kg; decreases ALP at 2 ml/kg after 60 days (14) 2019
EO's blend (10% THY, 10% CIN, others) 0, 100, 200, 400 mg/kg for 42 days Reduces total protein and plasma protein at 100 mg/kg; significant hematological changes (123) 2023
Dogs THEO Oral administration, 20% conc. No significant changes in biochemical parameters except serum urea levels (130) 2023
Broilers THY-CAR 0, 60, 100, 200 mg/kg diet Increases trypsin, lipase, and protease activities in 24-day-old birds; no effect in 42-day-old birds (125) 2013
THEO 0, 5, 7.5 g/kg diet Lowers serum protein, albumin, cholesterol, and triglyceride at 7.5 g/kg (131)
THEO 0.05–0.35 mg/kg Increases serum proteins and globulins; decreases ALT, albumin-to-globulin ratio, and urea; improves HDL levels (132) 2014
Nigella seed EOs 0.5–1.5 g/kg Decreases plasma total protein, albumin, ALT, and ALP; variable plasma urea and creatinine; reduces lymphocyte, monocyte, eosinophil, and heterophile counts (21) 2015
THY + BA THY: 100–200; BA: 1,000–2,000 mg/kg No effect on albumin, triglyceride, cholesterol, globulin, urea; increases lipase, lactase, and sucrose in jejunum (133)
THP 0.9% Significant effects on all serum constituents except total protein, albumin, and HDL (1) 2017
THEO 1–2 g/kg feed Increases plasma protein and globulin; decreases albumin/globulin ratio, and AST; enhances WBC (134)
THP 0, 2, 5, 8 g/kg Increases WBC, lymphocyte count, IgG, NO; decreases cholesterol, LDL; increases HDL (135)
THP 10–30 g/kg diet Increases plasma total protein, hemoglobin, and MCH with high doses (136) 2018
THEO 3 g/kg diet Improves HDL in aflatoxin- and ochratoxin-contaminated diets (137) 2019
THY and other THY-containing EOs Not specified Significant increase in eosinophils, lymphocytes, basophils, and monocytes % (p < 0.05) in all supplemented groups; higher WBC, total protein, albumin, lower cholesterol, and triglycerides (138) 2020
EOs blend with THY (main compound, 3.05%) 0, 50, 100, 200, 400 mg/kg Increases ileal sucrase activity (p < 0.05); quadratic increase in sucrase activity with EOs levels (19) 2021
THEO, OEO and other THY-containing E0's (star anise, rosemary) 25 mg/kg, THY = 1.02 mg/kg Increases trypsin, chymotrypsin, and elastase activities in EOs plus saponins group (139)
Laying hens THP 0, 3, 6, 9 g/kg diet Reduces triglycerides, LDL; lowers total cholesterol with 3 g/kg THP (128) 2015
THEO + peppermint EOs 100 mg/kg Decreases serum cholesterol; increases eggshell thickness and Haugh unit in cold-stressed hens (140) 2016
Quail THEO + savory EO 200–400 ppm Reduces triglycerides in both sexes; decreases cholesterol in males (141) 2018
THEO 400 ppm Decreases triglycerides in male birds at high concentrations (80) 2019
Ducks THEO 50–100 mg/kg No significant effect on serum protein, albumin, globulin, cholesterol, triglycerides, ALT, or AST (142) 2019
Pigeons THY 40 mg/kg bw for 15 days Decreases ALT and AST in THY-treated infected birds; no significant changes in albumin, creatinine, or electrolyte levels (143) 2020
Tilapia THEO 1% Enhances hematocrit, WBC, RBC, neutrophil, and monocyte counts (144) 2014
Rainbow trout THEO and fennel EO blend 10 ml·100 g−1 (1-week supplementation) Enhance bactericidal activity, increase total protein, albumin, cholesterol, triglyceride, and bilirubin; decrease plasma glucose; increase K, Na, Ca, and Mg levels; decrease Cl levels (145) 2013
THY 2–2.5 g Increases Hb, RBC, hematocrit, WBC at higher doses; enhances protease and lipase activities; no significant amylase changes (146) 2022
Tambaqui THEO (THY = 55.9%) 50 mg/L Loweres glucose levels post-anesthesia; increases glucose upon recovery (147) 2022
Common carp THP 0.5–2% Increases RBC, Hb, PCV, WBC; highest Hb, and PCV with 1.5% THP (90) 2017
THP (THY concentration not reported) 2% for 30 days Modulates erythrogram indices altered by lambda-cyhalothrin insecticide exposure; no significant differences in Hb, RBC count, PCV%, and MCV without insecticide (13) 2020
African catfish THEO 500 ppm for 1 month Reduces hepatorenal damage in thiamethoxam-intoxicated fish; partial restoration of serum hepatic enzymes and creatinine (148) 2020