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. 2025 Aug 12;12:1562641. doi: 10.3389/fvets.2025.1562641

Table 19.

Effects of THY- and THEO-based supplements on digestibility, fermentation, and fatty acid profile in various animal species.

Animal species Treatment/THY dose Duration Main findings References Year
Pigs EOs blend (18% THY and CIN, 0.01%) 35 days Increases dry matter and crude protein digestibility compared with control (18) 2012
THY (0.0067% or 0.0201%) 7 days No significant effect on fermentation products in the gut (168) 2014
EOs blend (13.5% THY and 4.5% CIN, 0.025%) 28 days Enhances apparent digestibility of dry matter, crude protein, and energy compared to control (121)
THY (100–200 mg/kg) + BA (1,000–2,000 mg/kg) 42 days Higher butyric acid concentrations and lower ammonia nitrogen in cecal content (133) 2015
Lippia origanoides-E. caryophyllata EOs (47.5% THY) N/A Increases cecal volatile fatty acids in treated groups (20) 2021
Dogs Yeast cell wall + OEO (0–3 kg/ton) 20 days Reduces dry matter digestibility, lowers fecal ammonia (17) 2023
Rabbits THY (250 mg/kg) 21 days Increases oleic, monounsaturated, and docosapentaenoic fatty acids in muscle (120) 2020
THY (0, 100, 200, and 300 g/T) Days 35–77 Increases Lactobacilli and decreases E. coli in cecal microbiota (172) 2021
Dairy
cows
TEHO and THY (50 mg/kg) 28 days No changes in volatile fatty acids or NH3 (171) 2021
Goats THY (0–600 mg/L) 24 h Reduces total gas and methane production and improves rumen fermentation characteristics (173) 2020
Blue foxes THY (0, 100, 200, and 300 mg/kg) 30 days The addition of 100 mg/kg THY to the diet significantly increases organic matter digestibility and crude protein digestibility (150) 2024
Broiler chickens THY-CAR blend 1:1 (0, 60, 100, and 200 mg/kg) N/A Decreases saturated fatty acids and increases PUFAs in serum and thigh (125) 2013
THY (200 mg/kg) 0–32 days Tendency to decrease propionic and butyric acid concentrations in cecal content, with no effect on molar ratios of short-chain fatty acids (22) 2014
Encapsulated phytogenic additive (100 mg/kg) 42 days Increases digestibility of crude protein in ileum (162) 2016
EO blend with saponins (THY at 1 mg/kg) N/A Improves protein and amino acid digestibility in ileum; transcriptomic analyses indicate enhanced macromolecule uptake and metabolism (163) 2017
THEO (0.05–0.1%) Days 0–28 No significant differences in fatty acid profile of breast muscle (59) 2019
Encapsulated THTY-CAR (0–120 mg/kg) N/A Decreased volatile fatty acids in coccidiosis-challenged chickens (165) 2020
EOs blend (50–400 mg/kg) 42 days Higher digestibility of dry matter, gross energy, and ether extract at all levels; crude protein digestibility improves at high doses (19) 2021
Broiler chickens feed THY (400 mg/kg) N/A No changes in fatty acid composition (167) 2017
Quail THY (0.0016 mol/day) Incubation period Polyunsaturated fatty acids are provided to embryo at day 4 for synthesis/deposition in membranes (174) 2017
THY (0–6.25 g/kg) 28 days Decreases saturated and increases PUFAs in high-dose groups (71) 2019