Table 2.

Mutations, deletions and truncations of select proteins in E. coli BW25113 and E. coli 6146 derived mutants following the exposure to one and two NAMs and 1x MIC of ciprofloxacin

	Strains	Protein	Mutation(s)*	Fold increase in Cip MIC
BW25113 derived	BW_M1	SoxR	R20L	8
	BW_Ibf_M1	ND	ND	8
	BW_Apap_M1	MarR	E136D, deletion – Y137	16
	BW_Apap_M2	MarR	1 base pair deletion	4
	BW_Ator_M1	MarR	deletions (L75, D76)	8
	BW_Ibf/Met_M1	GyrA	D87Y	32
	BW_Ibf/Dic_M1	GyrA	S83L	64
	BW_Ibf/Frs_M1	MarR	L78R	8
		AcrR	T5N
	BW_Apap/Ibf_M1	GyrA	S83 deletion	32
		MarR	V84E
	BW_Apap/Met_M1	MarR	13 base pair deletion	16
		AcrR	T5N
6146 derived	6146_M1	MarR	180 base pair deletion	8
	6146_Ibf_M1	GyrA	3 base pair deletion (S83 deletion)	8
	6146_Frs_M1	GyrA	S83L	32
	6146_Ibf/Frs_M1	GyrA	D87G	32
		MarR	13 base pair deletion
	6146_Ibf/Apap_M1	MarR	2 base pair insertion	8
		AcrR	T5N

ND No mutations determined, Cip ciprofloxacin, MIC Minimum Inhibitory Concentration, Ibf ibuprofen, Met metformin, Dic diclofenac, Frs furosemide.