Table 1.
Results of phylogenetic and mutational analysis of HBV whole genome sequences isolated from HBsAg positive donor blood samples.
| No. | Genotype | length | HBV DNA (copies/3 µL) | PreS1/PreS2/S protein | Polymerase | X protein | precore/core protein | accession No. | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| preS1 | preS2 | S | deletion | DRM | core promoter | precore | core protein | ||||||
| T2 | A2 | 3221 bp | 4163982.8 | – | – | – | – | – | 1858T > C | LC851032 | |||
| T9 | A1 | 3179 bp | 147.4 | S17_F25del | G138_Y140del |
sM1K*** sF20S sR24K sI68T sC76Y sF85C sM103I sI110L sS113T |
G17_T18del S198_I206del S321_F323del |
– | – | – | 1858T > C |
cR152_D153del (2354_2359del) cS183P(2441 C > A) |
LC851033 |
| T11 | A2 | 3221 bp | 248090469.2 | – | – | – | – | – | – | – | 1858T > C | – | LC851034 |
| T12 | A2 | 3221 bp | 20 | – | – | – | – | – | – | – | – | – | LC851035 |
| T24 | A2 | 3221 bp | 1517.4 | – | – | – | – | – | – | – | 1858T > C | – | LC851036 |
| T26 | A2 | 3176 bp | 47.3 | – |
N123_R137del F141L |
sF20S sS167L sL216* |
V304_S318del | – | H94Y | 1653 C > T | – | cS183P (2441 C > A) | LC851037 |
| T32 | A2 | 3221 bp | 102.4 | – | – | – | – | – | – | – |
eM1*(1814 A > C)* 1858T > C 1862G > T |
LC851038 | |
| T5 | B1 | 3215 bp | 115.8 | – | – | – | – | – | I127M | 1754T > G | eW28* (1896G > A)* |
2002 C > T cS181P (2441T > C) |
LC851443 |
| T6 | B2 | 3215 bp | 151 | W4P | – |
sL21S sM133L sY161F |
– | – | I127V | 1752 A > G | eW28* (1896G > A)* |
1915 C > G cI97L (2189 A > C) |
LC851444 |
| T7 | B1 | 3215 bp | 63 | – | – | sA159V | – | rtA194T | I127T | 1753T > G | – |
2002 C > T cS181P (2441T > C) |
LC851445 |
| T8 | B1 | 3215 bp | 101.1 | – | – |
sL21S sG145A |
– | – | I127M |
1754T > G 1613G > A |
eW28* (1896G > A)* |
2002 C > T cS181P (2441T > C) |
LC851446 |
| T15 | B1 | 3215 bp | 158.6 | – | – | – | – | – | – | – | 1858T > C |
2002 C > T cS181P (2441T > C) |
LC851447 |
| T18 | B1 | 3215 bp | 367744.3 | – | – | sC76Y | – | – | I127M | 1754T > G | eW28* (1896G > A)* |
2002 C > T cS181P (2441T > C) |
LC851448 |
| T21 | B2 | 3215 bp | 14.9 | – | – | sL95W | – | – | K130M/V131I | 1762 A > T/1764G > A | 1858T > C |
1915T > G cI97F (2189 A > T) |
LC851449 |
| T25 | B1 | 3215 bp | 97.9 | – | – | sT126A | – | – | – | – | eW28* (1896G > A)* |
2002 C > T cS181P (2441T > C) |
LC851450 |
| T33 | B4 | 3215 bp | 89.6 | – | – | sK122R | – | – | – | – | eG29D (1899G > A) |
1915 C > G cI97L (2189 A > C) |
LC851451 |
| T1 | C2 | 3215 bp | 1.2 | – | – |
sLI68T sL95W |
– | – |
I127N K130M/V131I |
1674T > C 1753T > A 1762 A > T/1764G > A |
– | – | LC852214 |
| T4 | C2 | 3215 bp | 363754086.6 | – | – | – | – | – | – | – | – | – | LC852215 |
| T10 | C2 | 3215 bp | 50.5 | – | – |
sF20S sV96A sL98V sI126S |
– | – | – | 1613G > A | – |
cI97L (2189 A > C) cQ182* (2444 C > T*) |
LC852216 |
| T14 | C2 | 3215 bp | 4113.4 | – | – |
sF20S sL77R |
– | – | K130M/V131I | 1762 A > T/1764G > A | eG29D (1899G > A) |
1915G > A cI97L (2189 A > C) cL100I (2198 C > A) |
LC852217 |
| T16 | C2 | 3215 bp | 410.9 | – | – | – | – | – |
H94Y K130M/V131I |
1653 C > T 1762 A > T/1764G > A |
eW28* (1896G > A)* | cL60V(2078 C > G) | LC852218 |
| T17 | C2 | 3215 bp | 57.9 | H51Q | – | sV96A | – | – | – | – | – |
2159 A > G cI97L (2189 A > C) |
LC852219 |
| T19 | C2 | 3215 bp | 382.8 | – | – | sF80S |
H94Y K130M/V131I |
1653 C > T 1762 A > T/1764G > A |
– | – | LC852220 | ||
| T20 | C2 | 3215 bp | 222784834.2 | – | – | – | – | – | K130M/V131I | 1762 A > T/1764G > A | – | – | LC852221 |
| T22 | C2 | 3155 bp | 192.8 | – |
M1del S124_P142del |
sI68T | G299_G318del | – | – | – | – |
2002 C > A cI97F (2189 A > T) |
LC852222 |
| T23 | C2 | 3215 bp | 7614.1 | – | – | sF20S | – | – |
I127S K130M/V131I |
1753T > G 1762 A > T/1764G > A |
eW28* (1896G > A)* | – | LC852223 |
| T27 | C2 | 3215 bp | 61739067.4 | – | – | – | – | – | – | – | – | 2002 C > A | LC852224 |
| T28 | C2 | 3215 bp | 532 | H51Q | – | sI68T | – | – |
I127T K130M/V131I |
1753T > C 1762 A > T/1764G > A |
– |
2159 A > G cI97L (2189 A > C) |
LC852225 |
| T30 | C2 | 3215 bp | 130.1 | – | – | – | – | – | – | 1766 C > G | – | – | LC852226 |
| T31 | C2 | 3182 bp | 463,650 | – |
M120I *** L131_P142del |
– | A312_S322del | – | – | – | eW28* (1896G > A)* | cS181P (2441T > C) | LC852227 |
| T34 | C2 | 3215 bp | 184.3 | – | – | – | – | – | V131I |
1764G > A 1766 C > T |
– | cI97L (2189 A > C) | LC852228 |
| T29 | D2 | 3182 bp | 493.3 | – | – |
sV118T sT127P sV128A |
– | – | I127L |
1752 A > C 1766 C > G |
– | 1915T > A | LC852229 |
| T3 | A | 217 bp | 8.1 | N/A | N/A | sS167L | N/A | N/A | N/A | N/A | N/A | N/A | LC867244 |
***Start codon mutation.
DRM: drug-resistant mutation, VEM: vaccine-escape mutation, N/A: not applicable.